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11tris01kg chemical compound  (Valiant Co Ltd)

 
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    Valiant Co Ltd 11tris01kg chemical compound
    11tris01kg Chemical Compound, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/11tris01kg+chemical+compound/Tris+base%2C+molecular+biology+grade/10__7554_slash_elife__32340-309-248-246
    Average 96 stars, based on 195 article reviews
    11tris01kg chemical compound - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: 32340 17 of 29 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti-HLADR Miltenyi Biotec #130-111-789 Fluorochrome: PE Antibody anti-CD20 BD biosciences Fluorochrome: PE; clone H1 Antibody anti-CD10 BD biosciences Fluorochrome: APC, clone HI10a Antibody anti-CD27 BD biosciences Fluorochrome: PerCPCy5.5; clone L128 Antibody anti-IgM Miltenyi Clone PJ2-22H3 Antibody anti-IgG BD biosciences Fluorochrome: BV605; clone G18-145 Antibody anti-IgA Miltenyi Clone IS11-8E10 Antibody anti-CD4 eBioscience Fluorochrome: Pacific blue; clone OKT4 Antibody anti-CD45RA BD biosciences Fluorochrome: PerCPCy5.5, clone HI100 Antibody anti-CCR7 Sony Fluorochrome: FITC; clone G043H7 Recombinant DNA reagent pcDNA 3.1D/V5-His-TOPO vector Thermo Fisher Scientific #K4900-01 Sequence-based reagent IRF4-specific primer Thermo Fisher Scientific #Hs01056533_m1 Sequence-based reagent GUSB Thermo Fisher Scientific #4326320E Peptide, recombinant protein rhGM-CSF R and D System #CAA26822 Peptide, recombinant protein rhM-CSF R and D System #NP_757350 Peptide, recombinant protein IFN-γ Boehringer Ingelheim Imukin Peptide, recombinant protein rhIL4 R and D System #P05112 Commercial assay or kit Lipofectamine LTX kit Thermo Fisher Scientific #15338100 Commercial assay or kit Dual-Luciferase 1000 assay system kit Promega #E1980 Commercial assay or kit ZR RNA Microprep kit Zymo research #R1061 Commercial assay or kit High-Capacity RNA-tocDNA kit Thermo Fisher Scientific #R4387406 Commercial assay or kit TOPO TA cloning kit Thermo Fisher Scientific #K450001 Commercial assay or kit directional TOPO expression kit Thermo Fisher Scientific #K4900-01 Commercial assay or kit QuikChangeII XL SiteDirected Mutagenesis Kit Agilent Technologies #200522 Commercial assay or kit LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific #L34957 Chemical compound, drug Tris MP biomedicals #11TRIS01KG Chemical compound, drug HCl Sigma #H1758 Chemical compound, drug NaCl Sigma #S3014 Chemical compound, drug Triton X-100 Sigma #T8532 Chemical compound, drug EDTA MP biomedicals #11EDTA05M1 Chemical compound, drug protease inhibitors Complete Roche #04693116001 Chemical compound, drug Phosphatase inhibitor cocktail Roche #04906837001 Guérin et al. eLife 2018;7:e32340.

    Plasmid Preparation:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: 32340 17 of 29 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti-HLADR Miltenyi Biotec #130-111-789 Fluorochrome: PE Antibody anti-CD20 BD biosciences Fluorochrome: PE; clone H1 Antibody anti-CD10 BD biosciences Fluorochrome: APC, clone HI10a Antibody anti-CD27 BD biosciences Fluorochrome: PerCPCy5.5; clone L128 Antibody anti-IgM Miltenyi Clone PJ2-22H3 Antibody anti-IgG BD biosciences Fluorochrome: BV605; clone G18-145 Antibody anti-IgA Miltenyi Clone IS11-8E10 Antibody anti-CD4 eBioscience Fluorochrome: Pacific blue; clone OKT4 Antibody anti-CD45RA BD biosciences Fluorochrome: PerCPCy5.5, clone HI100 Antibody anti-CCR7 Sony Fluorochrome: FITC; clone G043H7 Recombinant DNA reagent pcDNA 3.1D/V5-His-TOPO vector Thermo Fisher Scientific #K4900-01 Sequence-based reagent IRF4-specific primer Thermo Fisher Scientific #Hs01056533_m1 Sequence-based reagent GUSB Thermo Fisher Scientific #4326320E Peptide, recombinant protein rhGM-CSF R and D System #CAA26822 Peptide, recombinant protein rhM-CSF R and D System #NP_757350 Peptide, recombinant protein IFN-γ Boehringer Ingelheim Imukin Peptide, recombinant protein rhIL4 R and D System #P05112 Commercial assay or kit Lipofectamine LTX kit Thermo Fisher Scientific #15338100 Commercial assay or kit Dual-Luciferase 1000 assay system kit Promega #E1980 Commercial assay or kit ZR RNA Microprep kit Zymo research #R1061 Commercial assay or kit High-Capacity RNA-tocDNA kit Thermo Fisher Scientific #R4387406 Commercial assay or kit TOPO TA cloning kit Thermo Fisher Scientific #K450001 Commercial assay or kit directional TOPO expression kit Thermo Fisher Scientific #K4900-01 Commercial assay or kit QuikChangeII XL SiteDirected Mutagenesis Kit Agilent Technologies #200522 Commercial assay or kit LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific #L34957 Chemical compound, drug Tris MP biomedicals #11TRIS01KG Chemical compound, drug HCl Sigma #H1758 Chemical compound, drug NaCl Sigma #S3014 Chemical compound, drug Triton X-100 Sigma #T8532 Chemical compound, drug EDTA MP biomedicals #11EDTA05M1 Chemical compound, drug protease inhibitors Complete Roche #04693116001 Chemical compound, drug Phosphatase inhibitor cocktail Roche #04906837001 Guérin et al. eLife 2018;7:e32340.

    Sequencing:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: 32340 17 of 29 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti-HLADR Miltenyi Biotec #130-111-789 Fluorochrome: PE Antibody anti-CD20 BD biosciences Fluorochrome: PE; clone H1 Antibody anti-CD10 BD biosciences Fluorochrome: APC, clone HI10a Antibody anti-CD27 BD biosciences Fluorochrome: PerCPCy5.5; clone L128 Antibody anti-IgM Miltenyi Clone PJ2-22H3 Antibody anti-IgG BD biosciences Fluorochrome: BV605; clone G18-145 Antibody anti-IgA Miltenyi Clone IS11-8E10 Antibody anti-CD4 eBioscience Fluorochrome: Pacific blue; clone OKT4 Antibody anti-CD45RA BD biosciences Fluorochrome: PerCPCy5.5, clone HI100 Antibody anti-CCR7 Sony Fluorochrome: FITC; clone G043H7 Recombinant DNA reagent pcDNA 3.1D/V5-His-TOPO vector Thermo Fisher Scientific #K4900-01 Sequence-based reagent IRF4-specific primer Thermo Fisher Scientific #Hs01056533_m1 Sequence-based reagent GUSB Thermo Fisher Scientific #4326320E Peptide, recombinant protein rhGM-CSF R and D System #CAA26822 Peptide, recombinant protein rhM-CSF R and D System #NP_757350 Peptide, recombinant protein IFN-γ Boehringer Ingelheim Imukin Peptide, recombinant protein rhIL4 R and D System #P05112 Commercial assay or kit Lipofectamine LTX kit Thermo Fisher Scientific #15338100 Commercial assay or kit Dual-Luciferase 1000 assay system kit Promega #E1980 Commercial assay or kit ZR RNA Microprep kit Zymo research #R1061 Commercial assay or kit High-Capacity RNA-tocDNA kit Thermo Fisher Scientific #R4387406 Commercial assay or kit TOPO TA cloning kit Thermo Fisher Scientific #K450001 Commercial assay or kit directional TOPO expression kit Thermo Fisher Scientific #K4900-01 Commercial assay or kit QuikChangeII XL SiteDirected Mutagenesis Kit Agilent Technologies #200522 Commercial assay or kit LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific #L34957 Chemical compound, drug Tris MP biomedicals #11TRIS01KG Chemical compound, drug HCl Sigma #H1758 Chemical compound, drug NaCl Sigma #S3014 Chemical compound, drug Triton X-100 Sigma #T8532 Chemical compound, drug EDTA MP biomedicals #11EDTA05M1 Chemical compound, drug protease inhibitors Complete Roche #04693116001 Chemical compound, drug Phosphatase inhibitor cocktail Roche #04906837001 Guérin et al. eLife 2018;7:e32340.

    TA Cloning:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: 32340 17 of 29 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti-HLADR Miltenyi Biotec #130-111-789 Fluorochrome: PE Antibody anti-CD20 BD biosciences Fluorochrome: PE; clone H1 Antibody anti-CD10 BD biosciences Fluorochrome: APC, clone HI10a Antibody anti-CD27 BD biosciences Fluorochrome: PerCPCy5.5; clone L128 Antibody anti-IgM Miltenyi Clone PJ2-22H3 Antibody anti-IgG BD biosciences Fluorochrome: BV605; clone G18-145 Antibody anti-IgA Miltenyi Clone IS11-8E10 Antibody anti-CD4 eBioscience Fluorochrome: Pacific blue; clone OKT4 Antibody anti-CD45RA BD biosciences Fluorochrome: PerCPCy5.5, clone HI100 Antibody anti-CCR7 Sony Fluorochrome: FITC; clone G043H7 Recombinant DNA reagent pcDNA 3.1D/V5-His-TOPO vector Thermo Fisher Scientific #K4900-01 Sequence-based reagent IRF4-specific primer Thermo Fisher Scientific #Hs01056533_m1 Sequence-based reagent GUSB Thermo Fisher Scientific #4326320E Peptide, recombinant protein rhGM-CSF R and D System #CAA26822 Peptide, recombinant protein rhM-CSF R and D System #NP_757350 Peptide, recombinant protein IFN-γ Boehringer Ingelheim Imukin Peptide, recombinant protein rhIL4 R and D System #P05112 Commercial assay or kit Lipofectamine LTX kit Thermo Fisher Scientific #15338100 Commercial assay or kit Dual-Luciferase 1000 assay system kit Promega #E1980 Commercial assay or kit ZR RNA Microprep kit Zymo research #R1061 Commercial assay or kit High-Capacity RNA-tocDNA kit Thermo Fisher Scientific #R4387406 Commercial assay or kit TOPO TA cloning kit Thermo Fisher Scientific #K450001 Commercial assay or kit directional TOPO expression kit Thermo Fisher Scientific #K4900-01 Commercial assay or kit QuikChangeII XL SiteDirected Mutagenesis Kit Agilent Technologies #200522 Commercial assay or kit LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific #L34957 Chemical compound, drug Tris MP biomedicals #11TRIS01KG Chemical compound, drug HCl Sigma #H1758 Chemical compound, drug NaCl Sigma #S3014 Chemical compound, drug Triton X-100 Sigma #T8532 Chemical compound, drug EDTA MP biomedicals #11EDTA05M1 Chemical compound, drug protease inhibitors Complete Roche #04693116001 Chemical compound, drug Phosphatase inhibitor cocktail Roche #04906837001 Guérin et al. eLife 2018;7:e32340.

    Expressing:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: 32340 17 of 29 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti-HLADR Miltenyi Biotec #130-111-789 Fluorochrome: PE Antibody anti-CD20 BD biosciences Fluorochrome: PE; clone H1 Antibody anti-CD10 BD biosciences Fluorochrome: APC, clone HI10a Antibody anti-CD27 BD biosciences Fluorochrome: PerCPCy5.5; clone L128 Antibody anti-IgM Miltenyi Clone PJ2-22H3 Antibody anti-IgG BD biosciences Fluorochrome: BV605; clone G18-145 Antibody anti-IgA Miltenyi Clone IS11-8E10 Antibody anti-CD4 eBioscience Fluorochrome: Pacific blue; clone OKT4 Antibody anti-CD45RA BD biosciences Fluorochrome: PerCPCy5.5, clone HI100 Antibody anti-CCR7 Sony Fluorochrome: FITC; clone G043H7 Recombinant DNA reagent pcDNA 3.1D/V5-His-TOPO vector Thermo Fisher Scientific #K4900-01 Sequence-based reagent IRF4-specific primer Thermo Fisher Scientific #Hs01056533_m1 Sequence-based reagent GUSB Thermo Fisher Scientific #4326320E Peptide, recombinant protein rhGM-CSF R and D System #CAA26822 Peptide, recombinant protein rhM-CSF R and D System #NP_757350 Peptide, recombinant protein IFN-γ Boehringer Ingelheim Imukin Peptide, recombinant protein rhIL4 R and D System #P05112 Commercial assay or kit Lipofectamine LTX kit Thermo Fisher Scientific #15338100 Commercial assay or kit Dual-Luciferase 1000 assay system kit Promega #E1980 Commercial assay or kit ZR RNA Microprep kit Zymo research #R1061 Commercial assay or kit High-Capacity RNA-tocDNA kit Thermo Fisher Scientific #R4387406 Commercial assay or kit TOPO TA cloning kit Thermo Fisher Scientific #K450001 Commercial assay or kit directional TOPO expression kit Thermo Fisher Scientific #K4900-01 Commercial assay or kit QuikChangeII XL SiteDirected Mutagenesis Kit Agilent Technologies #200522 Commercial assay or kit LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific #L34957 Chemical compound, drug Tris MP biomedicals #11TRIS01KG Chemical compound, drug HCl Sigma #H1758 Chemical compound, drug NaCl Sigma #S3014 Chemical compound, drug Triton X-100 Sigma #T8532 Chemical compound, drug EDTA MP biomedicals #11EDTA05M1 Chemical compound, drug protease inhibitors Complete Roche #04693116001 Chemical compound, drug Phosphatase inhibitor cocktail Roche #04906837001 Guérin et al. eLife 2018;7:e32340.

    Mutagenesis:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: 32340 17 of 29 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti-HLADR Miltenyi Biotec #130-111-789 Fluorochrome: PE Antibody anti-CD20 BD biosciences Fluorochrome: PE; clone H1 Antibody anti-CD10 BD biosciences Fluorochrome: APC, clone HI10a Antibody anti-CD27 BD biosciences Fluorochrome: PerCPCy5.5; clone L128 Antibody anti-IgM Miltenyi Clone PJ2-22H3 Antibody anti-IgG BD biosciences Fluorochrome: BV605; clone G18-145 Antibody anti-IgA Miltenyi Clone IS11-8E10 Antibody anti-CD4 eBioscience Fluorochrome: Pacific blue; clone OKT4 Antibody anti-CD45RA BD biosciences Fluorochrome: PerCPCy5.5, clone HI100 Antibody anti-CCR7 Sony Fluorochrome: FITC; clone G043H7 Recombinant DNA reagent pcDNA 3.1D/V5-His-TOPO vector Thermo Fisher Scientific #K4900-01 Sequence-based reagent IRF4-specific primer Thermo Fisher Scientific #Hs01056533_m1 Sequence-based reagent GUSB Thermo Fisher Scientific #4326320E Peptide, recombinant protein rhGM-CSF R and D System #CAA26822 Peptide, recombinant protein rhM-CSF R and D System #NP_757350 Peptide, recombinant protein IFN-γ Boehringer Ingelheim Imukin Peptide, recombinant protein rhIL4 R and D System #P05112 Commercial assay or kit Lipofectamine LTX kit Thermo Fisher Scientific #15338100 Commercial assay or kit Dual-Luciferase 1000 assay system kit Promega #E1980 Commercial assay or kit ZR RNA Microprep kit Zymo research #R1061 Commercial assay or kit High-Capacity RNA-tocDNA kit Thermo Fisher Scientific #R4387406 Commercial assay or kit TOPO TA cloning kit Thermo Fisher Scientific #K450001 Commercial assay or kit directional TOPO expression kit Thermo Fisher Scientific #K4900-01 Commercial assay or kit QuikChangeII XL SiteDirected Mutagenesis Kit Agilent Technologies #200522 Commercial assay or kit LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific #L34957 Chemical compound, drug Tris MP biomedicals #11TRIS01KG Chemical compound, drug HCl Sigma #H1758 Chemical compound, drug NaCl Sigma #S3014 Chemical compound, drug Triton X-100 Sigma #T8532 Chemical compound, drug EDTA MP biomedicals #11EDTA05M1 Chemical compound, drug protease inhibitors Complete Roche #04693116001 Chemical compound, drug Phosphatase inhibitor cocktail Roche #04906837001 Guérin et al. eLife 2018;7:e32340.

    Staining:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: 32340 17 of 29 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody anti-HLADR Miltenyi Biotec #130-111-789 Fluorochrome: PE Antibody anti-CD20 BD biosciences Fluorochrome: PE; clone H1 Antibody anti-CD10 BD biosciences Fluorochrome: APC, clone HI10a Antibody anti-CD27 BD biosciences Fluorochrome: PerCPCy5.5; clone L128 Antibody anti-IgM Miltenyi Clone PJ2-22H3 Antibody anti-IgG BD biosciences Fluorochrome: BV605; clone G18-145 Antibody anti-IgA Miltenyi Clone IS11-8E10 Antibody anti-CD4 eBioscience Fluorochrome: Pacific blue; clone OKT4 Antibody anti-CD45RA BD biosciences Fluorochrome: PerCPCy5.5, clone HI100 Antibody anti-CCR7 Sony Fluorochrome: FITC; clone G043H7 Recombinant DNA reagent pcDNA 3.1D/V5-His-TOPO vector Thermo Fisher Scientific #K4900-01 Sequence-based reagent IRF4-specific primer Thermo Fisher Scientific #Hs01056533_m1 Sequence-based reagent GUSB Thermo Fisher Scientific #4326320E Peptide, recombinant protein rhGM-CSF R and D System #CAA26822 Peptide, recombinant protein rhM-CSF R and D System #NP_757350 Peptide, recombinant protein IFN-γ Boehringer Ingelheim Imukin Peptide, recombinant protein rhIL4 R and D System #P05112 Commercial assay or kit Lipofectamine LTX kit Thermo Fisher Scientific #15338100 Commercial assay or kit Dual-Luciferase 1000 assay system kit Promega #E1980 Commercial assay or kit ZR RNA Microprep kit Zymo research #R1061 Commercial assay or kit High-Capacity RNA-tocDNA kit Thermo Fisher Scientific #R4387406 Commercial assay or kit TOPO TA cloning kit Thermo Fisher Scientific #K450001 Commercial assay or kit directional TOPO expression kit Thermo Fisher Scientific #K4900-01 Commercial assay or kit QuikChangeII XL SiteDirected Mutagenesis Kit Agilent Technologies #200522 Commercial assay or kit LIVE/DEAD Fixable Aqua Dead Cell Stain Kit Thermo Fisher Scientific #L34957 Chemical compound, drug Tris MP biomedicals #11TRIS01KG Chemical compound, drug HCl Sigma #H1758 Chemical compound, drug NaCl Sigma #S3014 Chemical compound, drug Triton X-100 Sigma #T8532 Chemical compound, drug EDTA MP biomedicals #11EDTA05M1 Chemical compound, drug protease inhibitors Complete Roche #04693116001 Chemical compound, drug Phosphatase inhibitor cocktail Roche #04906837001 Guérin et al. eLife 2018;7:e32340.



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